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Related Experiment Videos

GABA-induced intracellular Ca2+ elevation in the embryonic chick brainstem slice.

Yoko Momose-Sato1, Masae Kinoshita, Katsushige Sato

  • 1Department of Physiology, Tokyo Medical and Dental University Graduate School and Faculty of Medicine, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.

Neuroscience Letters
|November 7, 2006
PubMed
Summary

Gamma-aminobutyric acid (GABA) triggers intracellular calcium ([Ca2+]i) increases in embryonic chick brainstem slices. This GABA-induced calcium elevation is critical for central nervous system development.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cellular Physiology

Background:

  • Gamma-aminobutyric acid (GABA) is a primary inhibitory neurotransmitter in the mature central nervous system (CNS).
  • The role of GABA in early CNS development is complex and still under investigation.
  • Intracellular calcium ([Ca2+]i) signaling is crucial for neuronal development and function.

Purpose of the Study:

  • To investigate the effect of GABA on intracellular calcium ([Ca2+]i) levels in embryonic chick brainstem slices.
  • To determine the source of calcium influx evoked by GABA.
  • To understand the developmental implications of GABA-mediated calcium signaling.

Main Methods:

  • Calcium imaging using Ca Green-1 AM dye.
  • Preparation of 8-day embryonic chick brainstem slices.

Related Experiment Videos

  • Pressure ejection of GABA, glycine, and glutamate onto brainstem slices.
  • Experiments conducted in normal and calcium-free extracellular solutions.

    • Main Results:

    • GABA application evoked a detectable elevation in intracellular calcium ([Ca2+]i).
    • The GABA-induced [Ca2+]i elevation was dependent on extracellular calcium, indicating calcium influx.
    • Glycine and glutamate also induced [Ca2+]i elevation, but to a lesser extent than GABA.
    • GABA-induced light-scattering changes were previously shown to be independent of extracellular calcium.

    Conclusions:

    • GABA-induced intracellular calcium ([Ca2+]i) elevation in embryonic brainstem slices is primarily mediated by calcium influx through voltage-gated channels due to membrane depolarization.
    • This calcium signaling pathway activated by GABA may play a significant role in the developmental processes of the central nervous system (CNS).
    • GABA's role in early development may involve excitatory actions, distinct from its inhibitory role in the mature CNS.