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Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice
Published on: June 24, 2022
Animal models for disease: knockout, knock-in, and conditional mutant mice
David F LePage1, Ronald A Conlon
1Department of Genetics, Case Western Reserve University, Cleveland, OH, USA.
Methods in Molecular Medicine
|November 7, 2006
Summary
Detailed protocols for gene targeting in mice are provided, enabling the modeling of genetic diseases. This guide is useful for labs performing gene targeting and those collaborating with gene targeting services.
Area of Science:
- Genetics
- Genomics
- Molecular Biology
Background:
- Genetic diseases can be modeled using gene-targeted mice, including knockout, knock-in, and conditional mutants.
- Gene targeting is a crucial technique for creating these animal models.
Purpose of the Study:
- To provide detailed protocols for gene targeting in mice.
- To guide laboratories in performing gene targeting for disease modeling.
Main Methods:
- Gene targeting of embryonic stem cells, suitable for laboratories with tissue culture facilities.
- Collaborative approaches with gene targeting services are discussed.
Main Results:
- Detailed protocols for gene targeting are presented.
- The importance of understanding the entire process for successful outcomes is highlighted.
Conclusions:
- Gene targeting protocols are essential for creating genetically modified mice to study diseases.
- Collaboration and knowledge sharing are key for successful gene targeting, particularly when outsourcing parts of the process.
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