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Updated: Jul 19, 2026

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
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Targeted chromosome elimination from ES-somatic hybrid cells

Hiroyuki Matsumura1, Masako Tada, Tomomi Otsuji

  • 1Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.

Nature Methods
|November 7, 2006
PubMed
Summary

Researchers engineered stem cell genomes by developing a technology to eliminate specific chromosomes from hybrid cells. This method allows targeted removal of chromosomes, including those with key pluripotency genes like Nanog.

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