Related Experiment Video
Updated: Jul 19, 2026

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
Published on: November 20, 2018
Targeted chromosome elimination from ES-somatic hybrid cells
Hiroyuki Matsumura1, Masako Tada, Tomomi Otsuji
1Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Abstract:
To engineer a stem cell genome, we developed a technology for targeted elimination of chromosomes from mouse embryonic stem (ES)-somatic hybrid cells. Here we demonstrate the use of a universal chromosome elimination cassette (CEC) for elimination of a single embryonic stem cell (ESC)-derived chromosome 11 or 12, and also both copies of chromosome 6, which harbor pluripotency-associated genes including Nanog. We attribute hybrid-cell pluripotency to the expression of Nanog from the reprogrammed somatic-cell nuclei.
Related Concept Videos
In-vitro Mutagenesis
Targeted Cancer Therapies
There are several types of targeted therapies against specific...
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...

