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Updated: Jul 19, 2026

A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
Published on: January 14, 2017
[Establishment of Z-HL16C cell line.]
Background:
To establish and study the nature and the application of Z-HL16C cell line.
Methods:
The cell line was continuously passed, frozen stored and recovered. Its application was expanded and the cell type was identified.
Results:
The cell line had an epithelial-cell-like shape, the size appeared uniform, the cell boundary was distinct. It has been continuously passed, frozen stored and recovered for ten years. Its recovery rate was about 90%. It has been proved to be sensitive to the tested viruses which were enteroviruses (Polio, Cox, Echo), influenza viruses, parainfluenzaviruses, adenoviruses, measles virus. This cell line has been identified as a cancerization cell.
Conclusion:
The cell line Z-HL16C has been stably established, it has a broad spectrum in sensitivity for culturing viruses.

