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[Determination of galactooligosaccharide in syrups by high performance ion exchange chromatography method]
Jian-Wen Li1, Zhu Wang, Yue-Xin Yang
1Institute for Nutrition and Food Safety, China CDC, Beijing 100050, China.
Objective:
The gradient procedure was developed based on AOAC 2001.02 to determine galactooligosaccharide (soluble dietary fiber with low molecular weight) by high performance ion exchange chromatography with pulsed amperometric detection.
Methods:
In the present study, the modified gradient procedure was used to separate the mixed standard on a CarboPac PA1 (2mm x 250mm) anion exchange column. The galactooligosaccharide in syrups were quantified and the repeatability and reproducibility of the modified method were also evaluated.
Results:
Galactose, glucose, sucrose, fructose and lactose were successfully separated. The galacto oligosaccharides contents in syrups of intra-assay were (49.59 +/- 3.34), (46.55 +/- 2.61) and (56.26 +/- 3.13)g/100g, RSD value were 6.73%, 5.62% and 5.56% respectively. RSD value for galactooligosaccharide of inter-assay in syrup 1 was 4.73%.
Conclusions:
Galactose and glucose were separated well and galactose was determined more accurately. the repeatability and the reproducibility of the modified method were excellent. This method can be used for GOS determination.
