Related Experiment Video
Updated: Jun 29, 2026

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology
Published on: March 17, 2011
A ruthenium red-toluidine blue procedure for staining epoxy sections in the light microscopy
E Crivellato1, M Zweyer, M Basa
1Istituto di Anatomia Umana Normale, Facoltà di Medicina e Chirurgia, Universită di Trieste, Italy.
Abstract:
Ruthenium red (RR) has been widely used as a fixation additive for electron microscopy on the basis of its capacity to retain acid mucopolysaccharide residues of the cell surface coat. Little is known about the properties of this compound as a direct staining agent for epoxy-resin embedded material. In this study, semithin sections of Epon-infiltrated muscle tissue samples have been treated with 1% RR followed by counterstaining with 1% toluidine blue. This 2 step staining procedure has proven to be simple, rapid, and reliable and to give a dramatic improvement in image resolution and contrast. Thus, we believe that histochemical procedures employing RR may find in the future interesting applications for the direct staining of epoxy-resin embedded tissues.
Related Concept Videos
Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
Preparation of Samples for Electron Microscopy
Simple Staining Technique

