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Updated: Jul 19, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
Published on: March 7, 2018
Measuring the flexibility of immunoglobulin by gold nanoparticles
G Steven Huang1, Yu-Shiun Chen, Hsiao-Wei Yeh
1Institute of Nanotechnology and Center for Nano Science & Technology, National Chiao Tung University, Hsinchu, Taiwan, Republic of China. gstevehuang@mail.nctu.edu.tw
Abstract:
We measured the flexibility of Fab and Fc arms of immunoglobulin using gold nanoparticles (GNPs). Enzyme-linked immunosorbent assay was performed to measure the affinity of anti-5 nm GNP antiserum against various sizes of GNPs. The flexibility of Fc was also measured by electron microscopy. The restricted binding affinity indicated that only a very limited amount of freedom was allowed for the Fab-Fab hinge, while Fab-Fc showed a much larger degree of freedom.

