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Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme
Published on: April 12, 2013
Developmentally regulated expression of MSX1, MSX2 and Fgfs in the developing mouse cranial base
1Department of Biomedicine, University of Bergen, Bergen, Norway. Xuguang.Nie@biomed.uib.no
The Angle Orthodontist
|November 9, 2006
Summary
Fibroblast growth factor (Fgf) and muscle segment homeobox (Msx) gene expression in cranial base development differs from other skeletal sites. Msx1 was not detected, while Fgf genes showed varied localization patterns.
Area of Science:
- Developmental biology
- Molecular genetics
- Craniofacial development
Background:
- Cranial base development is crucial for skull formation.
- Understanding gene expression patterns aids in identifying developmental mechanisms.
Purpose of the Study:
- To investigate the expression patterns of Fgf and Msx genes.
- To elucidate their roles in cranial base development.
Main Methods:
- Antisense riboprobes synthesized via in vitro transcription.
- Radioactive in situ hybridization on embryonic mouse head sections.
Main Results:
- Msx2 expressed in perichondrium; Msx1 not detected in cranial base.
- Fgf1 in osteogenic cells; Fgf10 in occipital-vertebral joint (E13-E14).
- Fgf2, Fgf7, Fgf18 in perichondria; Fgf12 transiently in chondrocranium; Fgf9 in hypertrophic chondrocytes.
Conclusions:
- Fgf and Msx gene expression in the cranial base is distinct.
- These genes exhibit unique spatiotemporal expression profiles during development.

