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Related Experiment Videos

Cyclin B targets p34cdc2 for tyrosine phosphorylation.

L Meijer1, L Azzi, J Y Wang

  • 1CNRS, Station Biologique, Roscoff, France.

The EMBO Journal
|June 1, 1991
PubMed
Summary

Cyclin B binding to p34cdc2 recruits it for tyrosine phosphorylation, forming inactive M phase-promoting factor (MPF). This complex requires DNA replication completion for full MPF activation, crucial for cell cycle G2/M transition.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • M phase-promoting factor (MPF) is a universal intracellular factor regulating the G2/M cell cycle transition.
  • Inactive MPF comprises tyrosine-phosphorylated p34cdc2 and unphosphorylated cyclin Bcdc13.
  • Active MPF, a histone H1 kinase (H1K), results from p34cdc2 dephosphorylation and cyclin Bcdc13 phosphorylation during M phase.

Purpose of the Study:

  • To investigate the role of cyclin in the formation of the MPF complex.
  • To elucidate cyclin's role in the tyrosine phosphorylation of p34cdc2.
  • To utilize synchronous sea urchin eggs as a model system for these investigations.

Main Methods:

  • Observation of p34cdc2 tyrosine phosphorylation during the G2 phase in sea urchin eggs.
  • Analysis of p34cdc2 concentration changes relative to phosphorylation.
  • Investigation of the association between cyclin accumulation and p34cdc2 tyrosine phosphorylation using aphidicolin and emetine treatments.

Main Results:

  • Massive tyrosine phosphorylation of p34cdc2 occurs as cells transition from S to G2 phase.
  • p34cdc2 tyrosine phosphorylation is closely associated with unphosphorylated cyclin accumulation.
  • Cyclin B synthesis and binding recruit p34cdc2 for tyrosine phosphorylation, forming an inactive complex.

Conclusions:

  • Cyclin B binding is essential for p34cdc2 tyrosine phosphorylation, preceding MPF activation.
  • The inactive p34cdc2-cyclin B complex requires DNA replication completion for full MPF activity.
  • Findings confirm in vitro data on cyclin's role in MPF formation and activation.

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