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Xylem and Transpiration-driven Transport of Resources02:03

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Updated: Jul 19, 2026

Phloem Sap Sampling from Brassica napus for 3D-PAGE of Protein and Ribonucleoprotein Complexes
11:23

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Published on: January 9, 2018

Protein extraction from xylem and phloem sap.

Julia Kehr1, Martijn Rep

  • 1Max-Planck-Institute of Molecular Plant Physiology, Potsdam, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|November 10, 2006
PubMed
Summary

Plant transport systems, phloem and xylem, carry proteins, not just nutrients. This study details methods for isolating these plant sap proteins for further proteomic analysis.

Area of Science:

  • Plant biology
  • Proteomics

Background:

  • Phloem and xylem vessels are known to transport small nutrient molecules in plants.
  • The presence of proteins in these vascular transport fluids is a relatively recent and surprising finding.
  • The specific functions of most proteins found in phloem and xylem sap remain largely unknown.

Purpose of the Study:

  • To describe methodologies for the isolation and purification of proteins from plant xylem and phloem saps.
  • To facilitate subsequent proteomic analyses of these vascular-transported proteins.

Main Methods:

  • Collection of xylem sap from plant sources.
  • Collection of phloem sap from plant sources.
  • Protein purification techniques applied to plant saps.

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Main Results:

  • Successful isolation of proteins from both xylem and phloem sap.
  • Purified protein samples suitable for proteomic analysis were obtained.

Conclusions:

  • Proteins are integral components of plant vascular transport systems.
  • Established methods enable the study of protein functions within xylem and phloem transport.