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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Updated: Jul 19, 2026

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

Protein extraction from woody plants.

Christophe Plomion¹, Céline Lalanne

¹NRA, UMR BIOGECO, Equipe de Génétique, Cestas, France.

Methods in Molecular Biology (Clifton, N.J.)

|November 10, 2006

Summary

This study details a protein extraction protocol optimized for wood-forming tissues and other plant organs. The method ensures efficient protein recovery for downstream proteomic analysis in various plant species.

Area of Science:

Plant Biology
Proteomics
Biochemistry

Background:

Accurate protein extraction is crucial for plant proteomic studies.
Wood-forming tissues present unique challenges for protein isolation.

Purpose of the Study:

To develop and optimize a total protein extraction protocol for differentiating secondary xylem.
To validate the protocol's efficacy across diverse plant organs and species.

Main Methods:

Tissue powdering in liquid nitrogen.
Trichloroacetic acid-acetone (TCA-acetone) precipitation for protein extraction.
Solubilization in an optimized buffer for 2D gel electrophoresis.

Main Results:

Successful protein extraction from wood-forming tissues.

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Isolation and Compositional Analysis of Plant Cuticle Lipid Polyester Monomers

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Published on: November 22, 2015

Demonstrated applicability to various organs including root, leaf, pollen, bud, flower, cambium, and phloem.

Validated protocol across broadleaf (oak, poplar) and conifer (pine) species.

Conclusions:

The presented protocol is robust and versatile for plant protein extraction.
It enables comprehensive proteomic analysis in diverse plant tissues.
The method facilitates further research in plant science and biotechnology.