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Updated: Jul 18, 2026
Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
Published on: September 5, 2017
Correlations between radiation-induced double strand breaks and cell cycle checkpoints in X-irradiated NIH/3T3
Mickael J Cariveaua1, Gerhard W Kalmus, Roberta M Johnke
1Department of Physics, East Carolina University, Greenville, North Carolina, USA. cariveau@sri.org
Background:
To better understand the relationship between the formation of radiation-induced DNA double strand breaks (DSB) and cell cycle checkpoint activation, studies were conducted in the NIH/3T3 fibroblast cell line in order to establish correlations between the temporal appearance of gamma-H2AX foci (a DSB) and the expression of the cell cycle regulatory proteins, the cyclins, and their cyclin kinase inhibitor, p21.
Materials And Methods:
Immunocytochemistry was used to determine the expression of cyclin E, A, B1, p21, and the generation of DSB in NIH/3T3 cells exposed to 2 or 4 Gy X-irradiation.
Results And Discussion:
The data suggest that the G1/S- and S-phase delay (cyclin E and cyclin A protein levels) are dependent on the dose of radiation while the G2/M (cyclin B1 protein levels) delay is dependent on the quantity of DSB sustained by the irradiated cell.
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