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Updated: Jul 18, 2026

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
Published on: April 7, 2017
Constitutive expression of RbAp46 induces epithelial-mesenchymal transition in mammary epithelial cells
1Zhuhai Campus, Zunyi Medical College, Zhuhai, Guangdong 519041, PR China.
Background:
Epithelial-mesenchymal transition (EMT) is a component of several morphogenetic and organogenetic processes during embryonic development. EMT is a strictly regulated process and its aberrant regulation may contribute to cancer progression and metastasis. The retinoblastoma suppressor (Rb)-associated protein 46 (RbAp46) is a component of the histone-modifying and -remodeling complexes.
Materials And Methods:
Western blot and immunofluorescent assays with epithelial and mesenchymal markers and cell migration and invasion assays were employed.
Results:
Mammary epithelial cells, MCF10AT3B, that express recombinant RbAp46 exhibited an EMT associated with the down-regulation of epithelial markers and up-regulation of mesenchymal markers, as well as an increased migration and invasion phenotype.
Conclusion:
Rbap46 may be an important player in EMT during embryonic development. Dysregulated RbAp46 expression may contribute to cancer progression and metastasis.
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