Production of sepedonin by Sepedonium chrysospermum NT-1 in submerged culture

Strains of Sepedonium chrysospermum and the anamorph strain of Hypomyces chrysospermus (congruent with Apiocrea chrysosperma) were isolated and purified from parasitic filamentous fungi on the fruiting bodies of Boletaceae, such as the Gyroponus and Suillus genera in Japan, and identified from formations of conidia and chlamydospores. It is known that these strains produce sepedonin. S. chrysospermum NT-1 strain was selected from these strains and isolated. As the optimum medium (CY-1 medium), 0.1% yeast extract was added to the fruiting-body-forming medium (C medium) of Schizophyllum commune. After 8 days of growth on CY-1 medium, the yield of sepedonin was about 34 mg per 2 g of glucose added. This sepedonin seemed to inhibit the growth of various gram-positive and gram-negative bacteria, yeasts and molds.