Potato tuber proteomics: comparison of two complementary extraction methods designed for 2-DE of acidic proteins
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Updated: Jul 18, 2026
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
Pierre Delaplace1, Froukje van der Wal, Jean-François Dierick
1Plant Biology Unit, Gembloux Agricultural University, Gembloux, Belgium. delaplace.p@fsagx.ac.be
Abstract:
Two protein extraction procedures were tested in order to remove interfering compounds prior to 2-DE of potato tubers. These methods using SDS lysis buffer and phenol-phase extraction were compared regarding the quality of the resulting 2-D gel. While the resolution of SDS extracts on semipreparative gels seems better, both methods lead to similar extraction yields and total number of spots. The procedures are complementary regarding the Mr range of preferentially extracted proteins.
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