Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 18, 2026

Air Filter Devices Including Nonwoven Meshes of Electrospun Recombinant Spider Silk Proteins
09:51

Air Filter Devices Including Nonwoven Meshes of Electrospun Recombinant Spider Silk Proteins

Published on: May 8, 2013

RGD-functionalized bioengineered spider dragline silk biomaterial.

Elisabetta Bini1, Cheryl Wong Po Foo, Jia Huang

  • 1Department of Biomedical Engineering, Department of Chemistry, Bioengineering and Biotechnology Center, Tufts University, 4 Colby Street, Medford, Massachusetts 02155, USA.

Biomacromolecules
|November 14, 2006
PubMed
Summary

Bioengineered spider silk proteins, including variants with RGD cell-binding domains, show potential as biomaterial scaffolds. These recombinant silks effectively support human mesenchymal stem cell differentiation into bone-like tissue.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy, safety, and tolerability of chenodeoxycholic acid (CDCA) in adult patients with cerebrotendinous xanthomatosis (RESTORE): A randomized withdrawal, double-blind, placebo-controlled, crossover phase-3 study.

Genetics in medicine : official journal of the American College of Medical Genetics·2025
Same author

First-in-human in vivo genome editing via AAV-zinc-finger nucleases for mucopolysaccharidosis I/II and hemophilia B.

Molecular therapy : the journal of the American Society of Gene Therapy·2022
Same author

Improvement of Local Cell Delivery Using Helix Transendocardial Delivery Catheter in a Porcine Heart.

International heart journal·2017
Same author

Manganese-Enhanced Magnetic Resonance Imaging Enables In Vivo Confirmation of Peri-Infarct Restoration Following Stem Cell Therapy in a Porcine Ischemia-Reperfusion Model.

Journal of the American Heart Association·2015
Same author

Genomic Analysis of the Emergence and Rapid Global Dissemination of the Clonal Group 258 Klebsiella pneumoniae Pandemic.

PloS one·2015
Same author

Arrayed Hollow Channels in Silk-based Scaffolds Provide Functional Outcomes for Engineering Critically-sized Tissue Constructs.

Advanced functional materials·2014

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Protein Engineering

Background:

  • Spider silk proteins possess exceptional mechanical properties, making them promising biopolymers for biomaterial scaffold development.
  • Bioengineering spider silk variants with cell-binding domains can enhance their utility in tissue regeneration.

Purpose of the Study:

  • To engineer and characterize recombinant spider silk proteins with RGD cell-binding domains.
  • To evaluate the efficacy of these engineered silks as biomaterial scaffolds for osteogenic differentiation of human bone marrow stromal cells.

Main Methods:

  • Cloning and expression of two bioengineered protein variants from Nephila clavipes dragline silk.
  • Characterization using Circular Dichroism (CD) and Fourier-Transform Infrared Spectroscopy (FTIR).

More Related Videos

Nanofibrillar Basement Membrane Mimic Made of Recombinant Functionalized Spider Silk in Custom-Made Tissue Culture Inserts
06:17

Nanofibrillar Basement Membrane Mimic Made of Recombinant Functionalized Spider Silk in Custom-Made Tissue Culture Inserts

Published on: November 1, 2024

Related Experiment Videos

Last Updated: Jul 18, 2026

Air Filter Devices Including Nonwoven Meshes of Electrospun Recombinant Spider Silk Proteins
09:51

Air Filter Devices Including Nonwoven Meshes of Electrospun Recombinant Spider Silk Proteins

Published on: May 8, 2013

Nanofibrillar Basement Membrane Mimic Made of Recombinant Functionalized Spider Silk in Custom-Made Tissue Culture Inserts
06:17

Nanofibrillar Basement Membrane Mimic Made of Recombinant Functionalized Spider Silk in Custom-Made Tissue Culture Inserts

Published on: November 1, 2024

  • Culturing human bone marrow stromal cells on recombinant silk films and fibers under osteogenic stimulation.

    • Main Results:

    • Engineered silks demonstrated structural transitions to beta-sheet structures upon methanol treatment.
    • Both native and RGD-modified recombinant spider silks supported enhanced osteogenic differentiation of human mesenchymal stem cells (hMSCs) compared to tissue culture plastic.
    • The recombinant spider silk without RGD showed superior bone-related outcomes (calcium deposition) compared to the RGD-modified version.

    Conclusions:

    • Bioengineered spider silk proteins hold significant potential for creating novel biomaterial scaffolds for bone tissue engineering.
    • Subtle modifications, such as the inclusion and presentation of RGD domains, critically influence cell-specific outcomes.
    • This study highlights the importance of silk source and RGD presentation in optimizing biomaterial performance for tissue regeneration.