Related Experiment Video
Updated: Jul 18, 2026

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
Published on: August 13, 2010
Functional class switch recombination may occur 'in vivo' in Waldenström macroglobulinaemia
Patricia Martín-Jiménez1, Ramón García-Sanz, María E Sarasquete
1Servicio de Hematología y Hemoterapia, Unidad de Biología Molecular, Hospital Universitario de Salamanca, Salamanca, Spain.
Abstract:
Waldenström macroglobulinaemia (WM) malignant cells have been considered incapable of undergoing class switch recombination (CSR). However, we report a WM patient who developed an IgG M-component 4 years after diagnosis. When the second monoclonal component appeared, reverse transcription-polymerase chain reaction showed the presence of pre (Cmu) and postswitch (Cgamma) clonotypic isotypes; sequencing of these isotypes demonstrated that both corresponded to the single clone amplified at diagnosis, including the same complementarity-determining region 3 and somatic mutation pattern. This proves that WM cells can undergo a functional in vivo CSR.
Related Concept Videos
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Differentiation of Common Myeloid Progenitor Cells
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Transcytosis of IgG
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
Viral Recombination
Protein Complexes with Interchangeable Parts
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

