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Assessing Changes in Volatile General Anesthetic Sensitivity of Mice after Local or Systemic Pharmacological Intervention
Published on: October 16, 2013
High throughput modular chambers for rapid evaluation of anesthetic sensitivity
Yi Sun1, Jingqiu Chen, Gregory Pruckmayr
1Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, Philadelphia, USA. yi.sun@uphs.upenn.edu
BMC Anesthesiology
|November 14, 2006
Summary
This study introduces a high-throughput system for precise anesthetic phenotyping in mice, measuring both induction and emergence. The new apparatus allows for more accurate assessment of anesthetic sensitivity, crucial for genetic studies.
Area of Science:
- Pharmacology
- Neuroscience
- Genetics
Background:
- Anesthetic sensitivity is a complex trait influenced by multiple genes.
- Traditional anesthetic phenotyping methods focus primarily on induction, neglecting emergence.
- High-throughput, precise behavioral screens are needed to dissect genetic contributors to anesthetic response.
Purpose of the Study:
- To develop and validate a novel, high-throughput apparatus for simultaneous anesthetic phenotyping of multiple mice.
- To accurately measure anesthetic sensitivity, including both induction and emergence parameters.
- To enable precise genetic dissection of anesthetic responsiveness.
Main Methods:
- A controlled environment apparatus was designed for simultaneous phenotyping of 24 mice.
- Loss of righting reflex was used to determine anesthetic-induced unconsciousness.
- Dose-response curves were fitted to calculate MAC(LORR) (EC50), Hill coefficients, and emergence times.
Main Results:
- Established MAC(LORR) values for halothane (0.79%), isoflurane (0.91%), and sevoflurane (1.96%) in C57BL/6J mice.
- Determined Hill coefficients for halothane (24.7), isoflurane (19.2), and sevoflurane (33.1).
- Measured emergence times: halothane (16.00 min), isoflurane (6.19 min), and sevoflurane (2.15 min) after 2.5 MAC(LORR) x hr exposure.
Conclusions:
- The developed system provides higher precision in assessing inhaled anesthetic responsiveness compared to previous methods.
- The apparatus is adaptable for delivering inhaled therapeutics or toxins and monitoring vital signs.
- This automated system facilitates future phenotyping of genetically modified mice to study anesthetic induction and emergence.
