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[Cigarette smoke and bronchoepithelium].

H Kawada1, Y Kudo, T Takizawa

  • 1First Department of Internal Medicine, Tokyo Women's Medical College, Japan.

Nihon Kyobu Shikkan Gakkai Zasshi
|February 1, 1991
PubMed
Summary

Active oxygen compounds in cigarette smoke contribute to reduced ciliary beat frequency (CBF). Antioxidant enzymes like superoxide dismutase (SOD) and catalase partially protect against smoke-induced ciliostasis in tracheal epithelium.

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Area of Science:

  • Respiratory Physiology
  • Toxicology

Background:

  • Cigarette smoke contains irritants and active oxygen species (e.g., O2-, H2O2).
  • These components can reduce ciliary beat frequency (CBF), impacting mucociliary clearance.
  • The specific role of active oxygen compounds in smoke-induced ciliostasis is not fully understood.

Purpose of the Study:

  • To investigate the contribution of active oxygen species in cigarette smoke to the inhibition of ciliary beat frequency (CBF).
  • To evaluate the protective effects of antioxidant enzymes against smoke-induced ciliostasis.

Main Methods:

  • Cultured rabbit tracheal epithelium was used in vitro.
  • Ciliary beat frequency (CBF) and time to ciliostasis were measured using a microphoto-oscillation technique.
  • Cigarette smoke extract was prepared and tested with and without superoxide dismutase (SOD) and catalase.

Main Results:

  • Cigarette smoke extract (10% and 20%) caused ciliostasis in 37±6 min and 17±2 min, respectively.
  • Smoke extract with SOD and catalase showed reduced toxicity, with ciliostasis occurring at 62±5 min (10%) and 24±1 min (20%).
  • Inactivated SOD and catalase did not prevent smoke-induced ciliostasis, indicating the enzymes' activity was crucial.

Conclusions:

  • Active oxygen compounds in cigarette smoke play a significant role in inhibiting ciliary beat frequency (CBF).
  • Superoxide dismutase (SOD) and catalase can mitigate the cilio-inhibitory effects of cigarette smoke extract.
  • These findings highlight the potential therapeutic targets for managing smoke-induced respiratory dysfunction.

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