Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glomerular dysfunction in nephrotic humans with minimal changes or focal glomerulosclerosis.

A Guasch1, H Hashimoto, R K Sibley

  • 1Department of Medicine, Stanford University Medical Center, California 94305.

The American Journal of Physiology
|May 1, 1991
PubMed
Summary

Glomerular filtration barrier dysfunction in nephrotic syndrome, particularly focal segmental glomerulosclerosis (FSGS), involves reduced restrictive pores and increased shunts, impairing kidney function. Minimal-change nephropathy (MCN) shows similar but less severe changes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Belatacept Combined With Transient Calcineurin Inhibitor Therapy Prevents Rejection and Promotes Improved Long-Term Renal Allograft Function.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2017
Same author

Postdepletion Lymphocyte Reconstitution During Belatacept and Rapamycin Treatment in Kidney Transplant Recipients.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
Same author

Renal transplantation using belatacept without maintenance steroids or calcineurin inhibitors.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2014
Same author

Imaging and elemental mapping of biological specimens with a dual-EDS dedicated scanning transmission electron microscope.

Ultramicroscopy·2013
Same author

Elastic and inelastic diffraction changes upon variation of the relative humidity environment of PurE crystals.

Acta crystallographica. Section D, Biological crystallography·2013
Same author

Towards on line monitoring the evolution of the myocardium infarction scar with an implantable electrical impedance spectrum monitoring system.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2013

Area of Science:

  • Nephrology
  • Renal Physiology
  • Glomerular Filtration

Background:

  • Nephrotic syndrome, characterized by proteinuria, arises from glomerular dysfunction.
  • Minimal-change nephropathy (MCN) and focal segmental glomerulosclerosis (FSGS) are common causes with distinct clinical courses.
  • Understanding the specific alterations in the glomerular filtration barrier is crucial for characterizing these diseases.

Purpose of the Study:

  • To characterize glomerular dysfunction in patients with MCN and FSGS using fractional clearances of uncharged dextrans.
  • To analyze the structural basis of impaired glomerular filtration in these nephrotic conditions.

Main Methods:

  • Measured fractional clearances (theta) of uncharged dextrans (28-60 A radii) in 34 nephrotic patients.
  • Applied a heteroporous membrane model to theoretically analyze dextran theta.

Related Experiment Videos

  • Correlated glomerular morphometry, specifically epithelial filtration slits, with filtration barrier function.

    • Main Results:

    • Both MCN and FSGS exhibited reduced restrictive pores and increased shuntlike pores, impairing size selectivity.
    • FSGS showed more severe alterations in pore structure and reduced glomerular filtration rate (GFR) compared to MCN.
    • Impaired charge selectivity contributed to proteinuria in MCN, while glomerular sclerosis reduced filtration surface in FSGS.

    Conclusions:

    • Glomerular filtration barrier dysfunction in nephrotic syndrome involves changes in pore size selectivity and number.
    • FSGS demonstrates more significant structural and functional impairments than MCN.
    • Glomerular morphometry correlates with the extent of membrane dysfunction, highlighting the role of filtration surface loss in FSGS.