Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Circular dichroism study of polyriboxanthylic acid.

M Spodheim-Maurizot, M Leng

    Nucleic Acids Research
    |March 1, 1977
    PubMed
    Summary

    Circular dichroism reveals three stable forms of poly(xanthosine) across pH and temperature variations. An electrostatic barrier explains observed hysteresis and metastability in poly(X) structures.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Molecular identification of Opisthorchis viverrini among the northeastern Cambodian population by internal transcribed spacer 2 based polymerase chain reaction.

    Tropical biomedicine·2024
    Same author

    Intestinal parasitic infections and risk factors among the population in Cambodia.

    Tropical biomedicine·2023
    Same author

    An international, open-label, randomised trial comparing a two-step approach versus the standard three-step approach of the WHO analgesic ladder in patients with cancer.

    Annals of oncology : official journal of the European Society for Medical Oncology·2022
    Same author

    [Research advances on the biomechanical micro- environment facilitated wound repair through the regulation of cell migration].

    Zhonghua shao shang yu chuang mian xiu fu za zhi·2022
    Same author

    Prevalence and intensity of helminths among inhabitants of the Chi River and Lahanna water reservoir areas of Northeastern Thailand.

    Tropical biomedicine·2021
    Same author

    The prevalence of intestinal helminth infection in rural subdistricts of northeastern Thailand.

    Tropical biomedicine·2021

    Area of Science:

    • Biophysical Chemistry
    • Polymer Science
    • Spectroscopy

    Background:

    • Poly(xanthosine) (poly(X)) exhibits complex structural behavior influenced by environmental factors.
    • Understanding the conformational states of polynucleotides is crucial for their biological function.

    Purpose of the Study:

    • To characterize the structural transitions of poly(X) using circular dichroism (CD) spectroscopy.
    • To investigate the influence of pH and temperature on poly(X) conformation.
    • To elucidate the mechanisms behind observed hysteresis and metastability in poly(X) solutions.

    Main Methods:

    • Circular dichroism (CD) spectroscopy was employed to analyze poly(X) at various pH and temperature conditions.
    • Hydrodynamic techniques were utilized to further investigate poly(X) behavior at basic pH.

    Main Results:

    • CD spectra indicated the existence of three stable conformational forms of poly(X) within the pH range of xanthosine protonation.
    • Hysteresis and metastability phenomena were observed in specific pH ranges, attributed to an electrostatic barrier.
    • Oligo(X) and poly(X) at basic pH were characterized using spectroscopic and hydrodynamic methods.

    Conclusions:

    • Poly(X) displays distinct structural polymorphism dependent on pH and temperature.
    • Electrostatic interactions play a significant role in the conformational dynamics and stability of poly(X).
    • The study provides insights into the structural versatility of xanthosine-based polymers.

    Related Experiment Videos