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Related Concept Videos

DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Transposons01:24

Transposons

Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...

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Related Experiment Video

Updated: Jul 18, 2026

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
10:09

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Published on: June 27, 2017

A multipurpose transposon-based vector system mediates protein expression in Rhodococcus erythropolis.

Khalid Ibrahim Sallam1, Noriko Tamura, Tomohiro Tamura

  • 1Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.

Gene
|November 14, 2006
PubMed
Summary

Researchers developed novel transposon-based vectors (pTNR-KA and pTNR-TA) for expressing Streptomyces proteasome in Rhodococcus erythropolis. This system enables efficient protein expression and functional proteasome production in a bacterial host.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • The proteasome is a crucial protein complex involved in cellular protein degradation.
  • Efficient expression systems are needed to study and utilize proteasomes from different organisms.
  • Streptomyces coelicolor proteasome has potential biotechnological applications.

Purpose of the Study:

  • To develop and validate novel transposon-based vectors for expressing the Streptomyces coelicolor proteasome in Rhodococcus erythropolis.
  • To demonstrate the functionality and biological activity of the expressed proteasome.

Main Methods:

  • Development of two transposon-based vectors, pTNR-KA and pTNR-TA.
  • Construction of expression cassettes containing Streptomyces proteasome genes (prcA and prcB) and regulatory elements.
  • Transposition of vectors into Rhodococcus erythropolis via electroporation.
  • Southern blot analysis to confirm gene integration and absence of interference.
  • Expression, isolation, and characterization of the Streptomyces proteasome.

Main Results:

  • Successful integration of transposon-based vectors into Rhodococcus erythropolis genome.
  • Confirmation that the integrated genes do not interfere with each other.
  • Demonstrated expression of functional Streptomyces proteasome in Rhodococcus erythropolis.
  • Confirmed peptidase activity of the isolated proteasome, indicating biological activity.

Conclusions:

  • The developed pTNR-KA and pTNR-TA vectors are effective tools for transposon-based protein expression in Rhodococcus species.
  • This system facilitates the production and study of heterologous protein complexes like the Streptomyces proteasome.
  • The successful expression and activity of Streptomyces proteasome in Rhodococcus erythropolis opens avenues for biotechnological applications.