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The tannin-osmium conductive staining after dehydration: an attempt to observe the chromosome structure by SEM
1Department of Anatomy, Tottori University School of Medicine, Yonago, Japan.
Journal of Electron Microscopy
|January 1, 1990
Abstract:
The tannin-osmium conductive staining method was modified in order to observe chromosomal structures without metal coating. The treatment with tannic acid and OsO4, which has been used in aqueous solution in the original method, was performed in acetone solution after dehydration in the present study. The usefulness of this method is discussed showing ultrastructural images of chromosomes of Drosophila and Tradescantia, which can be achieved by the method.