Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Cohesins02:20

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Condensins02:15

Condensins

Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering Nanoscale Frontiers: Valve Metal Oxide Nanostructures From Fundamentals to Multifunctional Biomedical Applications.

Chemical record (New York, N.Y.)·2026
Same author

Ancient and Emerging Nanostructures for Innovations to Fight Head and Neck Cancer.

Cells·2026
Same author

Facile immobilization of an unstable recombinant α-L-rhamnosidase on magnetite nanoparticles for efficient naringin biotransformation.

International journal of biological macromolecules·2026
Same author

Engineered Bacterial Biosynthesis of a Cysteine-Rich SPRR2A Fusion Protein with Dual Antibacterial and Anticancer Efficacy.

ACS synthetic biology·2026
Same author

A Head and Neck Cancer Spheroid Model for Phenotypic High-Content Screening of Chemoresistance.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Cyanostyryl-Guanidiniocarbonyl-Pyrrole Amphiphiles: From Aggregation-Induced Emission to Photodimerization, Self-Assembly, and Bioimaging.

ChemPlusChem·2025

Related Experiment Video

Updated: Jul 18, 2026

Isolating Interaction-Null/Impaired Mutants Using the Yeast Two-Hybrid Assay
02:44

Isolating Interaction-Null/Impaired Mutants Using the Yeast Two-Hybrid Assay

Published on: December 29, 2023

The Survivin-Crm1 interaction is essential for chromosomal passenger complex localization and function.

Shirley K Knauer1, Carolin Bier, Negusse Habtemichael

  • 1Georg-Speyer-Haus, Paul-Ehrlich-Strasse 42-44, Frankfurt D-60596, Germany.

EMBO Reports
|November 14, 2006
PubMed
Summary

The nuclear export receptor Crm1 tethers the chromosomal passenger complex (CPC) to centromeres via Survivin. Inhibiting this interaction disrupts CPC function during mitosis.

More Related Videos

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Related Experiment Videos

Last Updated: Jul 18, 2026

Isolating Interaction-Null/Impaired Mutants Using the Yeast Two-Hybrid Assay
02:44

Isolating Interaction-Null/Impaired Mutants Using the Yeast Two-Hybrid Assay

Published on: December 29, 2023

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The chromosomal passenger complex (CPC), comprising Aurora-B, Borealin, INCENP, and Survivin, is vital for mitosis.
  • Accurate CPC localization to centromeres is critical for chromosomal segregation and cytokinesis.

Purpose of the Study:

  • To investigate the role of the nuclear export receptor Crm1 in CPC localization.
  • To elucidate the interaction between Survivin and Crm1 in tethering the CPC to centromeres.

Main Methods:

  • Utilized leptomycin B treatment to inhibit Crm1.
  • Employed RNA-interference for Crm1 depletion.
  • Generated Survivin mutants lacking the nuclear export signal (NES).
  • Assessed CPC localization and function via genetic and cellular assays.

Main Results:

  • Crm1 interacts with a conserved leucine-rich NES in Survivin.
  • Inhibition or depletion of Crm1 prevents Survivin's centromeric targeting.
  • Mutating the Survivin NES disrupts CPC localization and mitotic function.
  • CPC assembly is independent of the Survivin-Crm1 interaction.

Conclusions:

  • The Survivin-Crm1 interaction is essential for tethering the CPC to centromeres.
  • Crm1 acts as a novel mitotic effector linking to the CPC.
  • This study reveals a critical interface for CPC-centromere tethering.