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Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
Published on: September 15, 2014
Axonal transport of BDNF precursor in primary sensory neurons
Hui Wang1, Linda Lin-Yan Wu, Xing-Yun Song
1Department of Human Physiology and Centre for Neuroscience, Flinders University of South Australia, GPO Box 2100 Adelaide, SA 5001, Australia.
Abstract:
Recent studies have shown that the precursor of brain-derived neurotrophic factor (pro-BDNF) activates p75NTR with high affinity to induce apoptosis. Here we show that pro-BDNF is transported anterogradely and retrogradely in sensory neurons of adult rats. After a crush injury of sciatic nerves, dorsal roots or dorsal column in adult Sprague-Dawley rats, the immunoreactivity for pro-BDNF accumulated at both the proximal and distal segments. The accumulation reached a maximum at 24 h after injury. Western blot analysis also revealed pro-BDNF in sciatic nerve segments proximal and distal to the ligature and in the spinal cord. Biotinylated or Alexa-488-labelled pro-BDNF injected into sciatic nerve was internalized and transported both retrogradely and anterogradely within sensory neurons. These results demonstrate that pro-BDNF is anterogradely and retrogradely transported in sensory neurons, suggesting that endogenous pro-BDNF may be released and play important functions.
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