Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Advances in corneal preservation.

R L Lindstrom1

  • 1University of Minnesota Department of Ophthalmology, Minneapolis.

Transactions of the American Ophthalmological Society
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vitrectomy and membrane dissection surgery.

Ophthalmology·2001
Same author

Two-year outcomes of intrastromal corneal ring segments for the correction of myopia.

Ophthalmology·2001
Same author

Astigmatism management: ablation patterns.

Current opinion in ophthalmology·2001
Same author

Refractive surgery in the new millennium.

Ophthalmology clinics of North America·2001
Same author

Intrastomal corneal ring segments: reversibility of refractive effect.

Journal of refractive surgery (Thorofare, N.J. : 1995)·2001
Same author

Laser in situ keratomileusis after intracorneal rings. Report of 5 cases.

Journal of cataract and refractive surgery·2001
Same journal

The Utility of Color Duplex Ultrasonography in the Diagnosis of Giant Cell Arteritis: A Prospective, Masked Study. (An American Ophthalmological Society Thesis).

Transactions of the American Ophthalmological Society·2018
Same journal

Autoimmune Retinopathy: Current Concepts and Practices (An American Ophthalmological Society Thesis).

Transactions of the American Ophthalmological Society·2018
Same journal

Erratum: Predictors of Intraocular Pressure After Phacoemulsification in Primary Open-Angle Glaucoma Eyes with Wide Versus Narrower Angles (An American Ophthalmological Society Thesis).

Transactions of the American Ophthalmological Society·2018
Same journal

The Relationship Between Ocular Itch, Ocular Pain, and Dry Eye Symptoms (An American Ophthalmological Society Thesis).

Transactions of the American Ophthalmological Society·2018
Same journal

The Effects of Phacoemulsification and Intraocular Lens Implantation on Anatomical and Functional Parameters in Patients with Primary Angle Closure: A Prospective Study. (An American Ophthalmological Society Thesis).

Transactions of the American Ophthalmological Society·2017
Same journal

Predictors of Intraocular Pressure After Phacoemulsification in Primary Open-Angle Glaucoma Eyes with Wide Versus Narrower Angles (An American Ophthalmological Society Thesis).

Transactions of the American Ophthalmological Society·2017
See all related articles

Researchers developed improved corneal storage media by creating in vitro models of human corneal endothelium (HCE) cells. These models help optimize preservation solutions to enhance corneal wound healing and reduce cell loss after transplantation.

Area of Science:

  • Ophthalmology
  • Tissue Engineering
  • Cell Biology

Background:

  • Maintaining endothelial function and corneal deturgescence are critical for corneal storage media.
  • Donor tissue quality, preservation time, and post-keratoplasty endothelial cell loss are key challenges.

Purpose of the Study:

  • To improve donor corneal tissue quality after 4°C storage.
  • To extend corneal preservation time and enhance wound healing.
  • To reduce endothelial cell loss post-keratoplasty.

Main Methods:

  • Developed in vitro human corneal endothelial (HCE) cell and epithelial cell culture models.
  • Utilized these models to study growth factors and medium components for corneal preservation solutions.
  • Investigated in vitro conditions to induce proliferation in quiescent HCE cells.

Related Experiment Videos

  • Developed a quantitative bioassay to measure DNA synthesis stimulation/inhibition.
  • Employed Scanning Electron Microscopy (SEM) to study extracellular matrix interactions.

    • Main Results:

    • In vitro models accurately reflect in vivo human corneal tissue responses.
    • Identified conditions promoting HCE cell proliferation and mitosis.
    • Demonstrated that extracellular matrix components modulate HCE cell growth, proliferation, and migration.
    • Developed a chondroitin sulfate-based medium supplemented with antioxidants and energy sources for 4°C preservation.

    Conclusions:

    • Optimized corneal storage media can improve donor tissue quality and post-transplant outcomes.
    • In vitro models are valuable tools for developing effective corneal preservation solutions.
    • Understanding HCE cell-extracellular matrix interactions is crucial for enhancing corneal wound healing and regeneration.