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Effects of poliovirus replication on undifferentiated and differentiated monocytic U937 cells: comparative studies
J A López-Guerrero1, C Cabañas, C Bernabeu
1Centro de Biología Molecular, Universidad Autónoma, CSIC, Madrid, Spain.
Abstract:
Poliovirus infection of either undifferentiated or differentiated U937 cells produced a decrease in the percentage of cells positive for the surface expression of the CD4, CD11c, CD14, or 8E11 antigens. The number of 4F2 surface molecules per cell increased in infected normal U937 cells, but was unaffected in differentiated cells. The level of O2- production in infected differentiated U937 cells was approximately 50% of that found when not infected. Finally, poliovirus RNA levels and infectious particle production were similar in either cell type.
Insights
Poliovirus infection alters immune cell markers and function. This study reveals how poliovirus impacts surface antigen expression and oxygen production in U937 cells, with implications for understanding viral pathogenesis.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- U937 cells are a human monocyte cell line widely used in immunological research.
- Poliovirus is a significant human pathogen that primarily affects the gastrointestinal tract and nervous system.
Purpose of the Study:
- To investigate the effects of poliovirus infection on U937 cell surface antigen expression and function.
- To compare the impact of poliovirus on undifferentiated versus differentiated U937 cells.
Main Methods:
- U937 cells (undifferentiated and differentiated) were infected with poliovirus.
- Flow cytometry was used to analyze surface antigen expression (CD4, CD11c, CD14, 8E11, 4F2).
- Oxygen radical production was measured in infected differentiated cells.
Main Results:
- Poliovirus infection decreased the expression of CD4, CD11c, CD14, and 8E11 on U937 cells.
- Increased 4F2 surface molecule expression was observed in infected normal U937 cells, but not differentiated cells.
- Oxygen production was reduced by approximately 50% in infected differentiated U937 cells.
- Poliovirus RNA and infectious particle production were comparable in both cell types.
Conclusions:
- Poliovirus infection modulates immune cell surface markers and impairs oxygen production in differentiated U937 cells.
- The differentiation state of U937 cells influences the cellular response to poliovirus infection.
- These findings contribute to understanding poliovirus-host interactions at the cellular level.