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CD59 functions as a signal-transducing molecule for human T cell activation
P E Korty1, C Brando, E M Shevach
1Laboratory of Immunology, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
Journal of Immunology (Baltimore, Md. : 1950)
|June 15, 1991
Summary
Antibodies targeting the CD59 antigen can activate human T lymphocytes, inducing proliferation and cytokine production when combined with co-stimulators. This activation is dependent on the CD3/TCR complex, highlighting CD59
Area of Science:
- Immunology
- Cell Biology
Background:
- CD59 antigen is a 20-kDa protein on leukocytes and RBCs, inhibiting complement-mediated cytolysis.
- CD59 shares structural similarities with murine Ly-6 antigens, suggesting potential T cell activation roles.
Purpose of the Study:
- Investigate if anti-CD59 monoclonal antibodies (mAbs) can activate human T lymphocytes.
- Determine the role of CD59 in T cell activation and signaling.
Main Methods:
- Utilized anti-CD59 mAbs targeting specific epitopes.
- Assessed T cell activation markers: intracellular calcium, inositol phosphate, IL-2 production, and proliferation.
- Employed CD59-loss mutants of Jurkat cells and anti-CD3 stimulation.
Main Results:
- Anti-CD59 mAbs induced T cell activation, including calcium flux, inositol phosphate production, IL-2 release, and proliferation, in the presence of co-stimulators.
- CD59-mediated activation required co-expression of the CD3/TCR complex.
- CD59 is not a downstream signal transducer for the TCR.
Conclusions:
- CD59 antigen plays multiple regulatory roles in the immune response.
- Anti-CD59 antibodies can trigger T cell activation pathways.
- CD59's function is linked to T cell receptor signaling but not as a downstream transducer.