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Human elongation factor 1 beta: cDNA and derived amino acid sequence
H von der Kammer1, J Klaudiny, M Zimmer
1Max-Planck-Institut für Biophysikalische Chemie, Abt. Molekulare Biologie, Göttingen, Germany.
Biochemical and Biophysical Research Communications
|May 31, 1991
Summary
Researchers identified a human elongation factor 1 beta (EF-1 beta) gene from ovarian cells. This gene shows high homology to other species, suggesting a conserved role in protein synthesis across vertebrates.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- Elongation factor 1 beta (EF-1 beta) is crucial for protein synthesis.
- Understanding human EF-1 beta provides insights into translational machinery.
Purpose of the Study:
- To identify and characterize the human EF-1 beta gene.
- To investigate the genomic organization and evolutionary conservation of human EF-1 beta.
Main Methods:
- cDNA library screening using lambda gt11.
- Subcloning into pUC18 and DNA sequencing.
- Northern and Southern blot analyses.
Main Results:
- A human cDNA clone, pHGP34, encoding a 225-amino acid protein homologous to EF-1 beta was identified.
- Northern analysis detected an EF-1 beta specific mRNA of 900 bp.
- Southern analysis suggested EF-1 beta is encoded by multiple genes in the human genome.
Conclusions:
- The identified clone pHGP34 represents human EF-1 beta.
- Human EF-1 beta exhibits significant sequence homology with orthologs in other species, including Artemia salina and Xenopus laevis.
- The human genome contains multiple genes for EF-1 beta, similar to EF-1 alpha.