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Updated: Jul 15, 2026
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
Published on: August 20, 2012
A cellular FRET-based sensor for beta-O-GlcNAc, a dynamic carbohydrate modification involved in signaling
Luz D Carrillo1, Lakshmipriya Krishnamoorthy, Lara K Mahal
1Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station, A5300 Austin, TX 78712-0265, USA.
Abstract:
beta-O-N-Acetyl-d-glucosamine (O-GlcNAc) is a dynamic carbohydrate modification that is involved in cell signaling and has been implicated in a variety of disease states, including Alzheimer's and type-II diabetes. Despite the importance of this modification, little is known about the spatial and temporal localization of O-GlcNAc during signaling. This is due to the lack of methods for the study of O-GlcNAc in living cell systems. Herein we report the first genetically encoded FRET-based sensor for the detection of O-GlcNAc dynamics in live mammalian cells.
