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Updated: Jul 18, 2026
Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice
Published on: February 22, 2019
Immunization with outer membrane proteins of Pasteurella multocida (6:B) provides protection in mice
S H Basagoudanavar1, D K Singh, B C Varshney
1Department of Biochemistry & Molecular Biology, Penn State College of Medicine, Hershey, PA 17033, USA.
Abstract:
The immunoprotective efficacy of Pasteurella multocida (6:B) outer membrane proteins (OMPs) was examined in the mouse model. Bacterial OMPs were extracted using sarkosyl method and analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting. Prototype vaccines were prepared using OMPs with adjuvants including dioleoyl phosphatidyl choline-based liposome and Montanide ISA206 water-in oil-in water emulsion. Antibody response to the vaccine was monitored using indirect enzyme linked immunosorbent assay. The results of the study showed that immunized mice had high titre with both the formulations. The vaccinated mice were able to survive a live virulent bacterial challenge. Based on the findings of the study it can be inferred that OMPs are important determinants of immunoprotection hence can serve as vaccine candidates against haemorrhagic septicaemia.
Insights
Outer membrane proteins (OMPs) from Pasteurella multocida (6:B) show immunoprotective efficacy in mice. These OMPs can be developed into effective vaccine candidates against haemorrhagic septicaemia.
Area of Science:
- Veterinary immunology
- Bacteriology
- Vaccine development
Background:
- Haemorrhagic septicaemia, caused by Pasteurella multocida, is a significant threat to livestock.
- Outer membrane proteins (OMPs) are crucial for bacterial-host interactions and are potential vaccine targets.
Purpose of the Study:
- To evaluate the immunoprotective efficacy of Pasteurella multocida (6:B) outer membrane proteins (OMPs) in a mouse model.
- To assess the potential of OMPs as vaccine candidates against haemorrhagic septicaemia.
Main Methods:
- Extraction and analysis of Pasteurella multocida (6:B) OMPs using sarkosyl method, SDS-PAGE, and immunoblotting.
- Development of prototype vaccines using OMPs with liposome and Montanide ISA206 adjuvants.
- Monitoring antibody response via indirect ELISA and challenging vaccinated mice with virulent bacteria.
Main Results:
- Both vaccine formulations induced high antibody titers in immunized mice.
- Vaccinated mice demonstrated significant survival rates against a live virulent bacterial challenge.
- OMPs were identified as key determinants of immunoprotection.
Conclusions:
- Pasteurella multocida (6:B) OMPs are effective in inducing an immunoprotective response.
- OMPs hold promise as viable vaccine candidates for preventing haemorrhagic septicaemia.
