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![Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F55858.jpg&w=3840&q=50)
Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
Published on: December 4, 2017
Spectroelectrochemistry of type II cytochrome c3 on a glycosylated self-assembled monolayer
Inês Gomes1, Roberto E Di, Patrícia M Pereira
1REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
Abstract:
A modified silver electrode was prepared by the self-assembly of a thiol-derivatized neoglycoconjugate, forming a 2D surface with maltose functionality. This self-assembled-monolayer-modified electrode was utilized for adsorption and spectroelectrochemical studies of tetraheme-containing type II cytochrome c3. The glycosylated surface allowed for the determination of the hemes' redox potentials and demonstrated enhanced spectroelectrochemical performance, in comparison to the widely used self-assembled monolayer of 11-mercapto-undecanoic acid.
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