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Related Experiment Videos

An endoplasmic reticulum-specific cyclophilin.

K W Hasel1, J R Glass, M Godbout

  • 1Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.

Molecular and Cellular Biology
|July 1, 1991
PubMed
Summary
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A novel protein, CPH2, acts as a peptidyl-prolyl cis-trans isomerase and binds cyclosporin A. Unlike other cyclophilins, CPH2 is located in the endoplasmic reticulum, suggesting a new mechanism for cyclosporin A

Area of Science:

  • Molecular Biology
  • Immunology
  • Biochemistry

Background:

  • Cyclophilin is a cytosolic enzyme inhibited by cyclosporin A, a key immunosuppressive drug.
  • The precise mechanisms of cyclosporin A's immunosuppressive action are not fully understood.
  • Existing knowledge focuses on cytosolic cyclophilins, leaving potential roles of other cellular compartments unexplored.

Purpose of the Study:

  • To identify and characterize novel cyclophilin-related proteins.
  • To investigate the cellular localization and enzymatic activity of newly discovered cyclophilins.
  • To explore the potential role of novel cyclophilins in cyclosporin A's mechanism of action.

Main Methods:

  • Degenerate oligonucleotide probing was used to isolate cDNA clones from human and mouse mRNA.

Related Experiment Videos

  • Bacterial expression and purification of the novel protein CPH2.
  • Enzymatic assays to determine peptidyl-prolyl cis-trans isomerase activity.
  • Cell fractionation and Western blot analysis to ascertain CPH2's subcellular localization.

    • Main Results:

    • A novel 20-kDa protein, CPH2, was identified, sharing 64% sequence identity with cyclophilin.
    • Bacterially expressed CPH2 exhibits cyclosporin A-binding and peptidyl-prolyl cis-trans isomerase activity.
    • CPH2 is exclusively localized to the endoplasmic reticulum, not the cytosol.

    Conclusions:

    • Cyclosporin A likely exerts its immunosuppressive effects through multiple cyclophilin family members.
    • The endoplasmic reticulum-localized CPH2 represents a novel target for cyclosporin A.
    • CPH2's role in preventing misfolding of T-cell membrane proteins may contribute to cyclosporin A's immunosuppression.