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Updated: Jul 18, 2026

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
Published on: June 26, 2016
Biology of circulating mRNA: still more questions than answers?
1Charité-Universitätsmedizin Berlin, Medizinische Klinik m.S. Onkologie u. Hämatologie, Molekularbiologisches Labor, Alte Apotheke, Schumannstr. 20-21, 10117 Berlin, Germany. michael.fleischhacker@charite.de
Abstract:
A few years after the first description of free-circulating DNA in plasma and serum, the detection of tumor-associated overexpressed mRNA in plasma was also reported. This observation has been confirmed and it seems to be clear that the presence of free-circulating RNA is a ubiquitous phenomenon. In this short review I will discuss some basic aspects of the release mechanisms for the RNA, its biological meaning, and clinical value.
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