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Updated: Jul 18, 2026

Analysis of Oxidative Stress in Zebrafish Embryos
Published on: July 7, 2014
Dialysis membrane-induced oxidative stress: role of heme oxygenase-1
Madhu Bhaskaran1, Neetu Radhakrishnan, Hitesh Patni
1Division of Kidney Diseases and Hypertension, North Shore University Hospital, Manhasset and Long Island Jewish Medical Center, New Hyde Park, NY, USA.
Abstract:
Dialysis membranes have been reported to induce monocyte apoptosis. We studied the role of oxidative stress in the induction of dialysis membrane-induced monocyte apoptosis. Superoxide dismutase, a superoxide scavenger, prevented dialysis membrane-induced monocyte apoptosis. Similarly, other antioxidants also inhibited dialysis membrane- induced apoptosis. In addition, the interaction of dialysis membranes with monocytes was associated with the generation of molecules leading to oxidative stress such as superoxide and TBARS. Interestingly, pre-induction of heme oxygenase (HO)-1 by hemin prevented dialysis membrane-induced monocyte apoptosis, whereas inhibition of HO-1 activity (treatment with tin protoporphyrin, SN-P) enhanced dialysis membrane-induced monocyte apoptosis. We suggest that oxidative injury associated with dialysis membrane and monocyte interaction plays a role in monocyte injury. Pre-induction of HO-1 may attenuate dialysis membrane-induced monocyte apoptosis.
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