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Time- and concentration-dependent effects of resveratrol in HL-60 and HepG2 cells
U Stervbo1, O Vang, C Bonnesen
1Department of Life Sciences and Chemistry, Roskilde University, Denmark.
Abstract:
Resveratrol, a phytochemical present in grapes, has been demonstrated to inhibit tumourigenesis in animal models. However, the specific mechanism by which resveratrol exerts its anticarcinogenic effect has yet to be elucidated. In the present study, the inhibitory effects of resveratrol on cell proliferation and apoptosis were evaluated in the human leukaemia cell line HL-60 and the human hepatoma derived cell line HepG2. We found that after a 2 h incubation period, resveratrol inhibited DNA synthesis in a concentration-dependent manner. The IC50 value was 15 microm in both HL-60 and HepG2 cells. When the time of treatment was extended, an increase in IC50 value was observed; for example, at 24 h the IC50 value was 30 microm for HL-60 cells and 60 microm for HepG2 cells. Flow cytometry revealed that cells accumulated in different phases of the cell cycle depending on the resveratrol concentration. Furthermore, an increase in nuclear size and granularity was observed in the G1 and S phases of HL-60 treated and HepG2-treated cells. Apoptosis was also stimulated by resveratrol in a concentration-dependent manner in HL-60 and HepG2 cells. In conclusion, resveratrol inhibits cell proliferation in a concentration- and time-dependent manner by interfering with different stages of the cell cycle. Furthermore, resveratrol treatment causes stimulation of apoptosis as well as an increase in nuclear size and granularity.
Insights
Resveratrol, a compound found in grapes, effectively inhibits cancer cell growth and promotes cell death. This study reveals its concentration and time-dependent effects on cell cycle progression and apoptosis in leukemia and liver cancer cells.
Area of Science:
- * Molecular Biology
- * Cancer Research
- * Pharmacology
Background:
- * Resveratrol, a natural phytochemical from grapes, shows anti-tumorigenic properties in animal models.
- * The precise mechanisms of resveratrol's anticarcinogenic effects require further investigation.
- * Understanding these mechanisms is crucial for developing novel cancer therapies.
Purpose of the Study:
- * To investigate the inhibitory effects of resveratrol on cell proliferation and apoptosis.
- * To evaluate these effects in human leukemia (HL-60) and hepatoma (HepG2) cell lines.
- * To elucidate the impact of resveratrol on cell cycle progression.
Main Methods:
- * Cell proliferation inhibition was assessed using DNA synthesis assays.
- * Resveratrol's effects on cell cycle distribution were analyzed via flow cytometry.
- * Apoptosis induction was measured in HL-60 and HepG2 cells following resveratrol treatment.
Main Results:
- * Resveratrol inhibited DNA synthesis in a concentration-dependent manner, with IC50 values of 15 µM at 2 h and increasing with longer incubation times.
- * Cell cycle analysis showed resveratrol-induced accumulation in specific phases, with increased nuclear size and granularity observed.
- * Apoptosis was significantly stimulated by resveratrol in both cell lines in a concentration-dependent manner.
Conclusions:
- * Resveratrol inhibits cancer cell proliferation through concentration- and time-dependent mechanisms.
- * It interferes with various stages of the cell cycle, leading to growth inhibition.
- * Resveratrol effectively stimulates apoptosis and alters nuclear morphology in cancer cells.
