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Assessment of pluripotency and multilineage differentiation potential of NTERA-2 cells as a model for studying human

Rajarshi Pal1, Geeta Ravindran

  • 1Embryonic Stem Cell Group, Reliance Life Sciences Pvt. Ltd., Dhirubhai Ambani Life Sciences Center, R-282, TTC Industrial Area of MIDC, Thane-Belapur Road, Rabale, Navi Mumbai 400 701, India. rajarshi_pal@relbio.com

Cell Proliferation
|November 18, 2006
PubMed

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The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...