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Updated: Jul 18, 2026

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Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models
Published on: April 26, 2024
Surface microhardness of enamel after different home bleaching procedures
Catharina Zantner1, Nils Beheim-Schwarzbach, Konrad Neumann
1Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Campus Benjamin Franklin, Charité-Universitätsmedizin Berlin, Berlin, Germany. catharina.zantner@charite.de
Summary
Home bleaching significantly impacts enamel microhardness, with sodium chlorite-based agents causing the most critical reductions. Different bleaching agents and concentrations affect enamel surface hardness.
Area of Science:
- Dental Materials Science
- Biomaterials
- Surface Chemistry
Background:
- Home bleaching procedures are widely used for aesthetic dental improvements.
- Understanding the effects of these treatments on tooth structure is crucial for clinical practice.
- Enamel microhardness is a key indicator of surface integrity and resistance to degradation.
Purpose of the Study:
- To evaluate how various home bleaching protocols influence the surface microhardness of human enamel.
- To compare the effects of different bleaching agents, concentrations, and application times.
Main Methods:
- 192 human incisors were divided into eight groups, including a control.
- Baseline enamel microhardness (Knoop) was measured.
- Enamel surfaces were treated for 14 days with various bleaching agents (carbamide peroxide, hydrogen peroxide, sodium chlorite) at different concentrations and durations.
- Microhardness was reassessed immediately after bleaching and after 6 weeks of storage in artificial saliva.
Main Results:
- Significant reductions in enamel microhardness were observed in groups treated with Colgate Simply White (5.9% hydrogen peroxide), Blend-a-med White Strips (5.9% hydrogen peroxide), and Odol-med3 Beauty-Kur (sodium chlorite).
- The most substantial decrease in microhardness occurred with the sodium chlorite-containing agent.
- These reductions were significant immediately after treatment and, in the case of the sodium chlorite group, persisted after storage.
Conclusions:
- Both the type and concentration of bleaching agents significantly affect enamel microhardness.
- Bleaching agents containing sodium chlorite, particularly when combined with citric acid, appear to be the most detrimental to enamel surface integrity.
- Clinicians should consider the potential impact of different home bleaching products on dental hard tissues.
