Related Experiment Video

Updated: Feb 13, 2026

Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation
04:10

Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation

Published on: October 20, 2023

2.3K

Remarkable immunostimulation effects of hybrid liposomes on human peripheral blood mononuclear cells in vitro

Yuji Komizu1, Yuka Tomonaga, Koichi Goto

  • 1Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.

Bioorganic & Medicinal Chemistry Letters
|November 18, 2006
PubMed

Immunostimulation effects of hybrid liposomes (HL25) composed of 90 mol% l-alpha-dimyristoylphosphatidylcholine (DMPC) and 10 mol% polyoxyethylene(25)dodecyl ether (C12(EO)25) on the normal human peripheral blood mononuclear cells (hPBMCs) were investigated in vitro. The concentrations of cytokines (IFN-gamma, IL-12) were significantly increased in the presence of HL25 ([DMPC] = 100 microM, [C12(EO)25] = 10 microM) and the maximum values attained were 13-14 times higher compared with those of control, though the viability and proliferation of hPBMCs were decreased under the same conditions. Such a remarkably high cytokine-production induced by liposomes without any stimulators was obtained for the first time. Furthermore, fluorescence microscopic and flow cytometric analyses indicated that the HL25 could be incorporated into hPBMCs by the membrane fusion and/or the endocytosis. These results suggest that HL25 should induce the effective immunopotentiation by stimulating the cytokine productivity of hPBMCs.

More Related Videos

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells05:38

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

Published on: February 12, 2021

4.8K
Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors09:45

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Published on: January 1, 2017

11.4K

Related Concept Videos

Hybridization of Atomic Orbitals I03:24

Hybridization of Atomic Orbitals I

The mathematical expression known as the wave function, ψ, contains information about each orbital and the wavelike properties of electrons in an isolated atom. When atoms are bound together in a molecule, the wave functions combine to produce new mathematical descriptions that have different shapes. This process of combining the wave functions for atomic orbitals is called hybridization and is mathematically accomplished by the linear combination of atomic orbitals. The new orbitals that...
67.8K
Hybrid Zones02:29

Hybrid Zones

Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
22.0K
Hybridization of Atomic Orbitals II03:35

Hybridization of Atomic Orbitals II

sp3d and sp3d 2 Hybridization
49.3K
Buffer Effectiveness02:19

Buffer Effectiveness

Buffer solutions do not have an unlimited capacity to keep the pH relatively constant . Instead, the ability of a buffer solution to resist changes in pH relies on the presence of appreciable amounts of its conjugate weak acid-base pair. When enough strong acid or base is added to substantially lower the concentration of either member of the buffer pair, the buffering action within the solution is compromised.
The buffer capacity is the amount of acid or base that can be added to a given volume...
55.6K
Framing Effects03:26

Framing Effects

Information is everywhere and its presentation—such as how and when items are presented—can impact our perceptions and decisions surrounding the info. This broad concept umbrellas framing effects—influences that occur due to the way information is framed in its appearance, whether it’s purely the order or the specific wording of a message. Let’s take a look at numerous ways in which two versions of something can objectively say the same thing, yet we respond in...
8.0K
In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
10.6K