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Related Experiment Videos

High-content screening of functional genomic libraries.

Daniel R Rines1, Buu Tu, Loren Miraglia

  • 1Genomics Institute of the Novartis Research Foundation, San Diego, CA, USA.

Methods in Enzymology
|November 18, 2006
PubMed
Summary

High-content screening (HCS) advances functional genomics in mammalian cells. This guide details preparing libraries and performing genome-wide HCS for molecular and phenotypic analysis.

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Area of Science:

  • Genomics
  • Cell Biology
  • Biotechnology

Background:

  • Functional genomics studies in mammalian cells have advanced significantly.
  • High-throughput methods for transfection, viral propagation, and library production (cDNA, siRNA) are now established.
  • Sensitive assay detection and instrumentation have improved.

Purpose of the Study:

  • To provide a comprehensive guide for functional genomics library preparation.
  • To detail the performance of high-content screening (HCS) at the genome level.
  • To highlight the application of HCS in mammalian cell-based studies.

Main Methods:

  • Utilizing automated microscopy and quantitative image analysis for high-content screening (HCS).
  • Developing and implementing high-throughput methodologies for genetic studies.

Related Experiment Videos

  • Curating and preparing large-scale functional genomics libraries (cDNA and siRNA).

    • Main Results:

    • High-content screening enables genome-wide analysis of molecular and phenotypic events in individual cells.
    • Automated microscopy and image analysis significantly facilitate functional genomics applications.
    • The described methods promise to expand the scope of functional genomic analyses.

    Conclusions:

    • High-content screening is a powerful technology for genome-wide functional genomics in mammalian cells.
    • Comprehensive library preparation and HCS are crucial for advancing cell-based studies.
    • This approach allows for detailed analysis of discrete molecular and phenotypic events.