Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extracellular Pgk1 or Its Derived Short Peptide Interacted with Membrane-Associated Enolase 2 Receptor: A Potential Therapy for ALS Motor Neuron Degeneration.

Biomolecules·2026
Same author

Administration of Pgk1 missense mutation leads to more effective mitigation of neurodegenerative effects observed in ALS mice and transgenic zebrafish.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Design and Preclinical Validation of an Eyeglass-Coupled Perilimbal Antenna for Retinal Prostheses With a Biocompatible Graft Technique.

Translational vision science & technology·2026
Same author

ENDOU-1-induced cytoplasmic HnRNPA3 recognizes m6A methylation on the upstream reading frame of human CHOP transcripts to achieve maximal CHOP translation.

Cellular and molecular life sciences : CMLS·2026
Same author

From enrichment to interpretation: PS4-driven reclassification in Taiwanese inherited retinal degeneration.

Human genomics·2026
Same author

Bortezomib Inhibits Cellular Proliferation and Inflammation in a Mouse Model of Proliferative Vitreoretinopathy.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Related Experiment Video

Updated: Jul 18, 2026

Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish
07:40

Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish

Published on: April 5, 2021

Egr1 gene knockdown affects embryonic ocular development in zebrafish.

Chao-Yu Hu1, Chang-Hao Yang, Wei-Yu Chen

  • 1Department of Ophthalmology, Far Eastern Memorial Hospital, Taipei, Taiwan.

Molecular Vision
|November 18, 2006
PubMed
Summary

Knocking down the early growth response factor 1 (Egr1) gene in zebrafish embryos disrupts ocular development, leading to smaller eyes and primitive ocular structures. This highlights Egr1

More Related Videos

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos
08:09

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos

Published on: July 19, 2010

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure
10:07

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure

Published on: March 20, 2012

Related Experiment Videos

Last Updated: Jul 18, 2026

Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish
07:40

Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish

Published on: April 5, 2021

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos
08:09

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos

Published on: July 19, 2010

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure
10:07

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure

Published on: March 20, 2012

Area of Science:

  • Developmental biology
  • Genetics
  • Ophthalmology

Background:

  • Early growth response factor 1 (Egr1) is a transcription factor involved in various cellular processes.
  • Understanding the role of specific genes in embryonic development is crucial for identifying causes of congenital abnormalities.

Purpose of the Study:

  • To investigate the impact of Egr1 gene knockdown on zebrafish embryonic ocular development.
  • To identify specific ocular defects caused by reduced Egr1 function using morpholino oligonucleotides (MO).

Main Methods:

  • Zebrafish embryos were injected with Egr1-specific morpholino oligonucleotides (MO) at various dosages.
  • Gross abnormalities, ocular dimensions, and specific cellular markers (glutamate receptor, acetylated alpha-tubulin, zpr-1) were analyzed.
  • Western blot and mRNA rescue experiments confirmed Egr1 knockdown effects.

Main Results:

  • Egr1 knockdown resulted in significant ocular defects, including smaller eyes and pupils.
  • Histological analysis revealed disorganized retinal cells, thin plexiform layers, and reduced numbers of retinal ganglion and photoreceptor cells.
  • Axonogenesis was prominently reduced, and lens development was impaired.

Conclusions:

  • The Egr1 gene is essential for normal zebrafish embryonic oculogenesis.
  • Egr1 knockdown leads to arrested development of ocular structures, resulting in microphthalmos.
  • Disruption of Egr1 function impacts lens and retinal differentiation.