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CyDye immunoblotting for proteomics: co-detection of specific immunoreactive and total protein profiles

Pamela M Donoghue1, Ciara A McManus, Niaobh M O'Donoghue

  • 1Proteome Research Centre, UCD Conway Institute of Biomolecular and Biomedical Research, Dublin, Ireland.

Proteomics
|November 18, 2006
PubMed

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.