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Updated: Jul 18, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
[Studies on aclacinomycin spectral characters and the interaction between aclacinomycin and DNA by spectroscopic
Min Hu1, Zhen-Xi Zhang, Guo-Li Shen
1The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Biomedical Engineering, Xi'an Jiaotong University, Xi'an 710049, China.
Abstract:
The interaction of aclacinomycin(ACR) and DNA was investigated by fluorescence spectrum, and the characteristics of the fluorescence and absorption of aclacinomycin (ACR) were studied. The results indicate that there are two situations: in the case that the concentration ratio of ACR to DNA is small, the ACR is intercalated into the stacked base pairs of DNA; in the other case that the concentration ratio of ACR to DNA is great, the interaction between ACR and DNA is complex. The binding constant of the interaction between ACR and DNA, calculated by the fluorescence titration method, is 2.7 x 10(6) mol x L(-1), and the binding site number is about 0.67 base pairs.
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