Related Experiment Video
Updated: Jul 18, 2026
09:40
Tropomodulin 3 Overexpression as a Marker for Platinum Resistance and Immune Infiltration in Ovarian Cancer
Published on: August 2, 2024
TRB3 interacts with CtIP and is overexpressed in certain cancers
1Department of Surgery, Zhongshan Hospital, Fudan University Medical School, 180 Fenglin Rd., Shanghai 200032, China.
Biochimica Et Biophysica Acta
|November 23, 2006
Summary
The study found that TRB3 protein interacts with CtIP, a cell cycle regulator, and is overexpressed in tumors. This interaction suggests TRB3
Area of Science:
- Molecular biology
- Cell biology
- Cancer research
Background:
- The protein TRB3 (human homolog of Drosophila Tribbles) negatively regulates Akt and S6 kinase activation.
- CtBP-interacting protein (CtIP) is a key regulator of the cell cycle and is implicated in tumor development.
Purpose of the Study:
- To investigate the interaction between TRB3 and CtIP.
- To explore the potential role of TRB3 in tumorigenesis through its interaction with CtIP.
Main Methods:
- Co-immunoprecipitation assays to confirm protein-protein interaction.
- Confocal microscopy to determine subcellular localization.
- Analysis of TRB3 expression in tumor tissues.
Main Results:
- TRB3 interacts with CtIP, specifically involving the C-termini of both proteins.
- TRB3 and CtIP co-localize in the nucleus of HeLa cells with a distinct dot-like pattern.
- TRB3 was found to be overexpressed in various human tumor tissues.
Conclusions:
- TRB3 interacts with the cell cycle regulator CtIP.
- The interaction and nuclear co-localization suggest a role for TRB3 in cell cycle control.
- TRB3 overexpression in tumors indicates its potential involvement in tumorigenesis via CtIP interaction.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
01:20The Retinoblastoma Gene
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
03:03mTOR Signaling and Cancer Progression
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
01:00Enzyme-linked Receptors
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
03:18Non-LTR Retrotransposons
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...