ADAMTS-13 levels in fresh, stored, and solvent detergent treated plasma

Area of Science:

• Hematology
• Biochemistry
• Plasma Products

Background:

• ADAMTS-13 is crucial in thrombotic thrombocytopenic purpura (TTP) pathophysiology.
• Plasma exchange efficacy may stem from removing harmful factors or supplying ADAMTS-13.
• Investigating plasma preparation methods is vital for TTP treatment.

Purpose of the Study:

• To evaluate the impact of storage conditions and processing methods on ADAMTS-13 levels in plasma.
• To determine if stored or treated plasma retains sufficient ADAMTS-13 for therapeutic use in TTP.

Main Methods:

• Fresh and fresh-frozen plasma (FFP) stored at 22°C were analyzed for ADAMTS-13 activity over 72 hours.
• ADAMTS-13 levels were measured in solvent detergent treated plasma (SDP) and cryosupernatant plasma (CSP).
• Total protein, albumin, fibrinogen, and immunoglobulin levels were also assessed.

Main Results:

• ADAMTS-13 activity remained consistent in fresh plasma stored for up to 48 hours.
• Vitex and Octapharma SDP showed comparable or slightly higher levels of ADAMTS-13 subunits.
• Cryosupernatant plasma (CSP) exhibited normal ADAMTS-13 levels, similar to untreated plasma.

Conclusions:

• Plasma storage at room temperature for up to 48 hours does not significantly reduce ADAMTS-13 levels.
• Both CSP and SDP maintain normal ADAMTS-13 levels, indicating their potential utility in TTP treatment.
• These findings support the use of processed plasma for managing TTP patients.