Related Experiment Video

Updated: Jul 18, 2026

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging
13:13

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

Published on: July 9, 2008

Discoidin domain receptor 2 is involved in the activation of bone marrow-derived dendritic cells caused by type I

Ji-Eun Lee1, Chon-Sik Kang, Xiu-Ying Guan

  • 1Department of Immunology, Chonbuk National University Medical School, Jeonbuk 561-180, Republic of Korea.

Biochemical and Biophysical Research Communications
|November 23, 2006
PubMed

Insights

Discoidin domain receptor 2 (DDR2) is crucial for activating mouse dendritic cells (DCs) by collagen. DDR2 signaling enhances DC functions, impacting immune responses.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Discoidin domain receptors (DDRs) are receptor tyrosine kinases that bind collagen.
  • Dendritic cells (DCs) are key antigen-presenting cells in the immune system.

Purpose of the Study:

  • To investigate the role of DDRs, specifically DDR2, in the activation of mouse bone marrow-derived dendritic cells (DCs) by type I collagen (ColI).

Main Methods:

  • Analysis of DDR2 expression in immature and mature DCs.
  • Treatment of DCs with ColI and assessment of DDR2 phosphorylation.
  • Evaluation of DC activation markers (IL-12, CD86) and functions (antigen uptake, T cell activation).
  • DDR2 depletion using siRNA.

Main Results:

  • DDR2 is constitutively expressed in immature DCs and upregulated in mature DCs.
  • ColI treatment phosphorylates DDR2, leading to increased IL-12 production, CD86 expression, and antigen uptake in immature DCs.
  • DDR2 depletion significantly reduced IL-12 and CD86 upregulation in ColI-treated DCs.
  • DDR2-ColI interaction enhances the capacity of mature DCs to activate allogeneic T cells.

Conclusions:

  • DDR2 acts as a critical collagen receptor mediating DC activation.
  • The DDR2-collagen interaction is vital for DC functional capacity and immune response regulation.

More Related Videos

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD22608:30

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Published on: May 10, 2022

Culture of myeloid dendritic cells from bone marrow precursors06:39

Culture of myeloid dendritic cells from bone marrow precursors

Published on: July 25, 2008

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
Inflammatory Bowel Disease III: Crohn's Disease01:25

Inflammatory Bowel Disease III: Crohn's Disease

Crohn’s disease is a chronic, relapsing form of inflammatory bowel disease characterized by segmental, transmural inflammation that can affect any part of the gastrointestinal tract. Its pathogenesis arises from a combination of genetic susceptibility, environmental exposures, epithelial barrier dysfunction, and immune dysregulation. Together, these factors lead to an exaggerated immune response against components of the gut microbiome.Genetic and Environmental InfluencesMultiple genetic...
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR activation may...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...