Electrochemical biosensor for catechol using agarose-guar gum entrapped tyrosinase

Area of Science:

• Biochemistry
• Electrochemistry
• Biosensor Technology

Background:

• Tyrosinase is a key enzyme involved in oxidation reactions.
• Electrochemical biosensors offer sensitive detection methods.
• Developing stable and reusable biosensors is crucial for practical applications.

Purpose of the Study:

• To construct an electrochemical biosensor for catechol determination.
• To utilize tyrosinase enzyme extracted from Amorphophallus companulatus.
• To immobilize the enzyme in a novel agarose-guar gum composite biopolymer matrix.

Main Methods:

• Enzyme extraction from Amorphophallus companulatus.
• Entrapment of tyrosinase in an agarose-guar gum composite biopolymer.
• Electrochemical detection of catechol via quinone reduction at -0.1 V vs Ag/AgCl.
• Linear sweep voltammetry for signal measurement.

Main Results:

• The biosensor demonstrated a linear response for catechol in the range of 6 x 10(-5) to 8 x 10(-4)M.
• A low detection limit of 6 microM was achieved.
• The biosensor exhibited reusability for up to 20 cycles.
• A shelf life exceeding 2 months was observed when stored at 4 degrees C.

Conclusions:

• The developed tyrosinase-based electrochemical biosensor is effective for sensitive catechol determination.
• The agarose-guar gum composite matrix provides a stable and reusable platform for enzyme immobilization.
• This biosensor shows potential for practical applications in catechol analysis.