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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
From DNA to Protein03:06

From DNA to Protein

The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...

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Related Experiment Video

Updated: Jul 18, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Maps, codes, and sequence elements: can we predict the protein output from an alternatively spliced locus?

Shalini Sharma1, Douglas L Black

  • 1Department of Microbiology, Immunology, and Molecular Genetics, Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, California 90095, USA.

Neuron
|November 23, 2006
PubMed
Summary

Predicting alternative splicing is challenging because regulatory proteins can act as enhancers or silencers. For the Nova protein, binding site location on pre-mRNA accurately predicts its splicing effects in neurons.

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An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

Related Experiment Videos

Last Updated: Jul 18, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

Area of Science:

  • Molecular Biology
  • Genetics
  • Neuroscience

Background:

  • Alternative splicing is regulated by protein interactions with cis-regulatory elements on pre-mRNA.
  • Predicting splicing outcomes from genomic sequence is complex due to the dual role (enhancer/silencer) of regulatory proteins.

Purpose of the Study:

  • To investigate the predictive power of splicing regulatory protein binding site location on alternative splicing outcomes.
  • To determine if the position of binding sites for the neuronal splicing regulatory protein Nova influences its function.

Main Methods:

  • Analysis of genomic sequence and pre-mRNA structures.
  • Computational prediction of splicing regulatory protein binding sites.
  • Experimental validation of predicted splicing effects.

Main Results:

  • The location of binding sites for the Nova protein is highly predictive of its effect on exon splicing.
  • Nova protein binding site proximity to splice sites correlates with its function as an enhancer or silencer.

Conclusions:

  • Splicing regulatory protein binding site positioning is a critical determinant of alternative splicing.
  • Understanding binding site location offers a more predictable model for Nova-mediated splicing regulation in neurons.