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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Updated: Jul 18, 2026

Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart
09:12

Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart

Published on: July 29, 2018

Specific RNAi mediated gene knockdown in zebrafish cell lines.

Jens Gruber1, Heiko Manninga, Thomas Tuschl

  • 1Department of Biochemistry, Max Planck Institute for Biophysical Chemistry, Goettingen, Germany.

RNA Biology
|November 23, 2006
PubMed
Summary

Zebrafish cell lines show efficient RNA interference (RNAi) with small interfering RNAs (siRNAs), leading to gene silencing. However, zebrafish embryos exhibit unspecific responses to siRNAs, causing developmental defects.

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Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart
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Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
11:33

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Published on: May 7, 2009

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • RNA interference (RNAi) is a powerful gene silencing mechanism.
  • Zebrafish (Danio rerio) are a key model organism in biological research.
  • Understanding RNAi efficiency in zebrafish cell lines and embryos is crucial for genetic studies.

Purpose of the Study:

  • To evaluate the efficiency of RNA interference in zebrafish cell lines (ZFL, SJD, ZF4).
  • To investigate the effects of small interfering RNAs (siRNAs) on zebrafish embryos.
  • To compare RNAi outcomes in zebrafish cell lines versus embryos.

Main Methods:

  • Transfection of zebrafish cell lines with siRNAs targeting lamin A, lamin B2, and Eg5.
  • Microinjection of siRNAs into zebrafish embryos.
  • Phenotypic analysis of treated cell lines and embryos.

Main Results:

  • High efficiency (nearly 100%) of gene silencing in zebrafish cell lines via microinjection of siRNAs.
  • Moderate efficiency (30%) of gene silencing in cell lines using cationic liposome transfection.
  • Zebrafish embryos injected with siRNAs showed morphological defects, developmental abnormalities, and high mortality, indicating unspecific responses.

Conclusions:

  • Zebrafish cell lines possess a fully functional RNA interference machinery.
  • Zebrafish embryos display unspecific responses to siRNA injection, unlike established cell lines.
  • RNAi is a viable tool for gene knockdown in zebrafish cell lines, but requires careful consideration in embryonic studies.