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G-protein Coupled Receptors

G-protein Coupled Receptors

G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.

G Protein-coupled Receptors

G Protein-coupled Receptors

G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...

Transducer Mechanism: G Protein–Coupled Receptors

Transducer Mechanism: G Protein–Coupled Receptors

G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical, 7TM, or...

G-protein Coupled Receptors

G-protein Coupled Receptors

G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.

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Related Experiment Video

Updated: Jul 18, 2026

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

Published on: June 9, 2017

Sensitive and specific method for detecting G protein-coupled receptor mRNAs.

Arne Hansen¹, Yidong Chen, Jason M Inman

¹Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institute of Health, Department of Health and Human Services, Bethesda, Maryland 20892, USA. arnehansen@nidcr.nih.gov

|November 23, 2006

Summary

Researchers developed a new method to detect gene expression for G protein-coupled receptors (GPCRs), even with limited RNA samples. This technique allows for better analysis of these crucial cell signaling proteins.

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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

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Last Updated: Jul 18, 2026

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

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Published on: June 9, 2017

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

Area of Science:

Molecular Biology
Cell Signaling
Genomics

Background:

G protein-coupled receptors (GPCRs) are vital cell surface receptors involved in numerous physiological processes.
Detecting GPCR gene expression is challenging due to low messenger RNA (mRNA) abundance.
Conventional microarray techniques struggle with quantifying scarce transcripts.

Purpose of the Study:

To develop a sensitive method for assaying GPCR gene expression.
To enable the study of GPCRs using limited total RNA samples.
To profile GPCR expression in human bone marrow stromal cells (BMSCs).

Main Methods:

Multiplex polymerase chain reaction (PCR) amplification of GPCR-encoding mRNAs.
Array-based detection of amplified DNA sequences (amplicons).
Assay validation using as little as 1 microgram of total RNA.

Main Results:

Successfully detected and quantified GPCR gene expression from minimal RNA input.
The method demonstrated high sensitivity for scarce mRNA targets.
GPCR expression profiles were successfully generated for three human BMSC lines.

Conclusions:

The developed multiplex PCR and array detection method is effective for GPCR gene expression analysis.
This technique overcomes limitations of conventional methods for scarce mRNA detection.
The findings provide insights into GPCR expression in human bone marrow stromal cells.